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SKU:C-39016

PromoCell GmbH

SupplementMix / Keratinocyte Growth Medium 2 1 mix

SupplementMix / Keratinocyte Growth Medium 2 1 mix

Special order item or temporarily out of stock.

Serum-free cell culture medium for keratinocytes from the epidermis.

Product description

Keratinocyte Growth Medium 2 is a serum-free medium developed for the cultivation of epidermal keratinocytes without feeder cells.

Although all  media are optimized for use with primary human cells, there is received feedback from customers that this particular medium can also be used for horse and porcine keratinocytes, as well as mouse and rat keratinocytes after adapting the CaCl₂ concentration. In addition, it is suited for rat corneal keratinocytes.

View a list of references where this medium was used with other species/cell types.

Components:

  • Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMix
  • Growth Medium Kit: Includes Basal Medium and SupplementPack
  • SupplementMix: Contains all media supplements premixed in one vial
  • SupplementPack: Contains all media supplements as individual vials

Additional information

Media supplement | Final supplement concentration
Bovine Pituitary Extract 0.004 ml/ml (30 µg/ml)
Epidermal Growth Factor (recombinant human) 0.125 ng/ml
Insulin (recombinant human) 5 μg/ml
Hydrocortisone 0.33 μg/ml
Epinephrine 0.39 μg/ml
Transferrin (recombinant human) 10 μg/ml
CaCl₂ 0.06 mM

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