SKU:740420.10
Macherey-Nagel
NucleoBond Xtra Midi EF, Midi kit for endotoxin-free plasmid DNA
NucleoBond Xtra Midi EF, Midi kit for endotoxin-free plasmid DNA
In stock
Product description
Product description
NucleoBond Xtra Midi EF is an anion exchange column kit for manual and automated use. Our 2nd generation anion exchange resin and column filters allow for a convenient and fast processing. The kit is capable of isolating high-purity, high-integrity plasmid DNA with exceptionally low endotoxin levels (≤ 0.05 EU/µg DNA), rendering it ideal for transfection and gene therapy applications.
Additional information
Additional information
Application Endotoxin-free plasmid DNA isolation
Selling unit 10 Prep(s), 50 Prep(s)
Target Plasmid DNA
CE certified No, research use only
Technology Anion exchange chromatography
Brand NucleoBond
Format Midi prep
Handling Gravity flow
Lysate clarification Column filter
Automated use Yes, can be automated on the Andrew+ pipetting robot
Sample material Bacteria, E. coli
Sample amount < 200 mL (high copy), < 400 mL (low copy)
Vector size < 300 kbp
Typical yield 500 µg
Theoretical binding capacity 1000 µg
Typical purity A260/A280 1.8−1.95
Endotoxin level ≤ 0.05 EU/µg DNA
Isopropanol precipitation Centrifugation, NucleoBond Finalizer, NucleoSnap Finisher Midi, NucleoSpin Finisher Midi
Preparation time 85 min/prep
Typical downstream application Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Shelf life (from production) 39 Month(s)
Hazardous material Yes
