SKU:740593
Macherey-Nagel
NucleoBond PC 10000, Giga kit for transfection-grade plasmid DNA
NucleoBond PC 10000, Giga kit for transfection-grade plasmid DNA
In stock
Product description
Product description
NucleoBond PC 10000 is an anion exchange column kit for manual and semi automated use. The AX 10000 column contains our 1st generation anion exchange resin. The kit is capable of isolating high-purity, high-integrity plasmid DNA with transfection grade quality. Depending on the sample input, yields of up to 10 mg are achievable.
Additional information
Additional information
Application High and low copy plasmid DNA purification
Target Plasmid DNA
CE certified No, research use only
Technology Anion exchange chromatography
Brand NucleoBond
Format Giga prep
Handling Gravity flow
Lysate clarification Folded filter
Automated use No
Sample material Bacteria, E. coli
Sample amount 500−2000 mL (high copy), 1−4 L (low copy)
Vector size < 300 kbp
Typical yield 2000−10000 µg
Theoretical binding capacity 10000 µg
Typical purity A260/A280 1.8−1.95
Endotoxin level 1−10 EU/µg DNA
Isopropanol precipitation Centrifugation, NucleoBond Finalizer, NucleoSnap Finisher Maxi, NucleoSpin Finisher Midi
Preparation time 120−150 min/2 preps
Typical downstream application Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Shelf life (from production) 39 Month(s)
Scope of delivery NucleoBond AX 10000 Columns, NucleoBond Folded Filters (Type 1 and 2), buffers, RNase A
Gross weight (incl. packaging) 6309 g / 13.91 lbs
Packaging dimensions 305 x 220 x 233 mm / 12.01 x 8.66 x 9.17 Inch
Hazardous material Yes
