Midori Green Direct 50µl sample

MIDORI Green Direct is a safe alternative to ethidium bromide for visualization of double-stranded DNA, single-stranded DNA, and RNA in agarose gels. In contrast to most other non-ethidium bromide based dyes, MIDORIGreen Direct is just added to your samples. The loading dye is already included.  You do not need to add any other loading dye to both gel matrix and running buffers

Direct Staining of DNA/RNA
No toxicity, non-carcinogenic
Safe alternative of Ethidium Bromide
Loading Dye is included
Very low Background

Get the best signal to noise ratio
The direct staining of the DNA rather than the gel eliminates the background staining providing a perfect signal (Fig. 1). MIDORIGreen Direct was developed to work with Blue Light LED illuminators, but you can also use it with a regular UV transilluminator.

Better Results for Downstream Application
The isolation of DNA from agarose gels enables downstream applications. It is well known that many dyes, such as ethidium bromide or even SYBR™ Green are strong enzyme inhibitors due to their intercalating properties. MIDORI Green dyes bind to the DNA backbone. This results in a much higher efficiency for downstream applications, i.e. cloning (Fig. 2), seqeuncing, PCR, etc.

Proven Safety
MIDORI Green Direct stain is non-carcinogenic and less mutagenic compared to ethidium bromide. Furthermore, we can state that MIDORI Green Direct is impenetrable to latex gloves and cell membranes.

MIDORI Green Direct is classified as non-hazardous to aquatic life, under CCR Title 22 regulation. Thus, small amounts of MIDORI Green Direct stain can be safely released into the environment.

Ames-Test
Cytotoxicity
Cell membrane Permeability
Hazardous Waste Screening
Latex and Nitrile Gloves Penetration

Loading Dye
MIDORI Green Direct stains are provided in a form of 10X sample loading dyes and they are to be added to your samples only. You do not need to add any other dyes to the gel matrix nor to the running buffers.