SKU:FG-LK30
Nippon Genetics Europe GmbH
FastGene Kickspeed DNA Ligation kit 30 rxns
FastGene Kickspeed DNA Ligation kit 30 rxns
Temporarily out of stock.
Ligation of DNA strands
Quick ligation of DNA fragments in 5 min at room temperature
Catalyses the formation of a phosphodiester bond for the connection of two DNA strands
T4 Ligase will join blunt ends and sticky ends
Supplied with optimal buffer conditions for an efficient ligation
Highest activity and purity
Product description
Product description
Kickspeed DNA Ligation Kit
FastGene® Kickspeed DNA Ligation kit is formulated for quick ligation of DNA fragments with cohesive ends within 5 min at room temperature. This product uses T4 DNA Ligase and 2X Kickspeed DNA Ligation buffer.
Characteristics
5 min ligation reaction with all types of DNA fragments
Reaction at room temperature
Rapid and efficient Kickspeed Ligation buffer
Applications
General cloning
Blunt-end cloning
TA cloning
Library construction
Linker ligation
T4 DNA Ligase
The FastGene® T4 DNA Ligase catalyzes the formation of a covalent bond between the 5′-phosphate and 3′-OH in nicked duplex DNA or at two DNA ends. This activity is very useful to ligate DNA fragments with either cohesive or blunt ends, that are generated by restriction enzyme digestion. T4 DNA Ligase can also ligate RNA with DNA or RNA in a double helix with low efficiency. The T4 DNA Ligase is cloned and expressed in E. coli, and purified to homogeneity. The Ligase is free of endonuclease, exonuclease and phosphatase.
Additional information
Additional information
