SKU:20-700-20
Sartorius Lab Instruments GmbH Co. KG
EZ-PCR Mycoplasma Detection kit
EZ-PCR Mycoplasma Detection kit
Special order item or temporarily out of stock.
The EZ-PCR™ Mycoplasma Detection Kit is a highly-sensitive and -specific PCR assay designed to test for the presence of over 90 species of Mycoplasma, Acholeplasma, and Spiroplasma in cell cultures with a detection limit of 10 CFU/mL.
The kit contains a ready-to-use PCR reaction mix with highly-optimized mycoplasma-specific primers, a positive control, and an internal control, all for the simple and efficient discovery of mycoplasma contamination.
Product description
Product description
EZ-PCR Mycoplasma Test Kit is designed to detect the presence of mycoplasma contaminating biological materials, such as cultured cells.
Mycoplasma detection by the direct culture procedure is time-consuming and some mycoplasma species are difficult to cultivate. With PCR testing, results are obtained within a few hours, since the presence of contaminant mycoplasma can be easily detected simply by verifying the bands of amplified DNA fragments in electrophoresis.
There is no need to prepare probes labeled with radioisotopes, or to calculate enzyme, dNTP’s or buffer concentrations. Instead, a ready-to-use, optimized PCR mix is supplied. The Reaction Mix contains a precipitant for direct loading of PCR products onto agarose gel. The primer set allows detection of various mycoplasma species (M.fermentans, M.hyorhinis, M.arginini, M.orale, M.salivarium, M.hominis, M.pulmonis, M.arthritidis, M.bovis, M.pneumoniae, M.pirum and M.capricolum), as well as Acholeplasma and Spir.
Additional information
Additional information
