NucleoSpin miRNA Mini kit for miRNA and RNA purification

Application Isolation of total RNA
Selling unit 10 Prep(s), 50 Prep(s), 250 Prep(s)
Target RNA
CE certified No, research use only
Technology Silica membrane technology
Brand NucleoSpin
Format Mini prep
Handling Centrifugation
Lysate clarification Centrifugation, NucleoSpin Filter
Automated use No
Sample material Animal tissue, Cells, Human tissue, Reaction mixture
Sample amount < 10⁷ cultured cells, < 30 mg human / animal tissue, < 50 mg plant tissue, < 150 µL reaction mixtures,
Fragment size > 18 nt
Typical yield 10 µg small RNA, 90 µg large RNA (10⁷ HeLa cells)
Theoretical binding capacity 200 µg
Elution volume 30–100 µL
Preparation time 45 min/6 preps (total RNA)
Typical downstream application Array technology, Northern blotting, Primer extension, qRT−PCR, RNA-Seq
Storage temperature 15–25 °C / 59–77 °F
Shelf life (from production) 24 Month(s)
Hazardous material Yes