NucleoSpin® Plasmid

Application High and low copy plasmid DNA purification
Selling unit 10 Prep(s), 50 Prep(s), 250 Prep(s)
Target Plasmid DNA
CE certified No, research use only
Technology Silica membrane technology
Brand NucleoSpin
Format Mini prep
Handling Centrifugation, vacuum
Lysate clarification Centrifugation
Automated use No
Sample material Bacteria, E. coli
Sample amount 1−5 mL (high copy), 6−10 mL (low copy)
Vector size < 25 kbp
Typical yield 25−45 µg
Theoretical binding capacity 60 µg
Typical purity A260/A280 1.8−1.85
Endotoxin level > 50 EU/µg DNA
Elution volume 50 µL
Preparation time 20 min/6 preps
Typical downstream application Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Storage temperature 15–25 °C / 59–77 °F
Shelf life (from production) 27 Month(s)
Hazardous material Yes