NucleoBond® Xtra Maxi

Application Transfection-grade plasmid DNA isolation
Selling unit 10 Prep(s), 50 Prep(s), 100 Prep(s)
Target Plasmid DNA
CE certified No, research use only
Technology Anion exchange chromatography
Brand NucleoBond
Format Maxi prep
Handling Gravity flow
Lysate clarification Column filter
Automated use No
Sample material Bacteria, E. coli
Sample amount < 600 mL (high copy), < 1200 mL (low copy)
Vector size < 300 kbp
Typical yield 1000 µg
Theoretical binding capacity 3000 µg
Typical purity A260/A280 1.8−1.95
Endotoxin level 1−10 EU/µg DNA
Isopropanol precipitation Centrifugation, NucleoBond Finalizer Large, NucleoSnap Finisher Maxi, NucleoSpin Finisher Midi
Preparation time 75 min/prep
Typical downstream application Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Storage temperature 15–25 °C / 59–77 °F
Shelf life (from production) 39 Month(s)
Hazardous material Yes