FastGene qFYR Real-Time PCR System

Highest precision instrument

The FastGene^® qFYR is a highest precision instrument for performing quantitative polymerase chain reaction (qPCR) experiments. qPCR is a well-established method for the sensitive detection and quantification of nucleic acids. During a measurement, the target DNA or RNA sequence is amplified, while a cycle-dependent increase of a fluorescent signal is detected in real-time.

The device for multiple applications

The FastGene^® qFYR was developed to meet highest laboratory standards and deliver reliable performances for various real-time qPCR applications:

• Gene expression analysis
• Absolute and relative quantification
• Endpoint qualitative analysis
• Genotyping
• Gene mutation analysis
• Pathogen detection
• GMO detection
• Protein stability screening
• miRNA analysis
• Melting curve analysis

Innovative Technology

High sensitivity optical design

The FastGene^® qFYR has a unique, patented optical detection system. It combines a high quality PMT (photomultiplier tube) with a Fresnel lens that has a short focal length. The resulting short distance from detector to sample reduces signal loss and cross-talk between samples, and generally improves signal sensitivity.

Superior thermal cycler

The FastGene^® qFYR delivers excellent temperature control, temperature precision (± 0.2 °C) and uniformity over the entire 96-well plate. The unique hollowed out thermal block design reduces its overall weight and ensures rapid heating and cooling rates (up to 6 °C per second) for fast qPCR protocols. The system also uses state-of-the-art Peltier components for highest reaction quality and performance stability, providing reliable and consistent qPCR results.

Fast scanning mode − 4+1 channels

4 different fluorescence channels and a double FAM/SYBR channel enabling fast measurements.

Due to the small distance between the scanning head and the plate, the FastGene^® qFYR has an outstanding level of sensitivity and virtually no cross talk between the individual wells.
Four different colour channels are installed in the scanning head to be able to excite all commonly used qPCR dyes in a single plate scan (8 sec).

The double FAM/SYBR channel allows to perform melt curve and high resolution melt experiments in fast dual mode, which halves the measuring time in this channel.

Channel 1: FAM/SYBR Green etc.
Channel 2: VIC/JOE/HEX/TET etc.
Channel 3: ROX/Texas Red etc.
Channel 4: Cy5 etc.
Channel 5: FAM/SYBR Green etc.