SKU-koodi:FG-LM50
Nippon Genetics Europe GmbH
FastGene Kickspeed 2X DNA Ligation Mix 50 rxns
FastGene Kickspeed 2X DNA Ligation Mix 50 rxns
Tilapäisesti loppu varastosta.
Ligation of DNA strands
Ready-to-use solution for 50 reactions
Quick ligation of DNA fragments in 5 min at room temperature
Catalyses the formation of a phosphodiester bond for the connection of two DNA strands
T4 Ligase will join blunt ends and sticky ends
Supplied with optimal buffer conditions for an efficient ligation
Highest activity and purity
Tuotteen kuvaus
Tuotteen kuvaus
Kickspeed 2x DNA Ligation Mix
The FastGene® Kickspeed 2X DNA Ligation Mix is a ready-to-use solution of T4 DNA Ligase for both sticky-end and blunt-end ligation reactions. This master mix enables quick ligation in a short incubation time (< 5min) at room temperature.
Characteristics
2X Master mix of T4 DNA Ligase and ligation enhancer
Fastest ligation of dsDNA with sticky end and blunt end
Incubation-free ligation
Applications
Vector construction
Linker ligation
Fragment assembly
Routine cloning
T4 DNA Ligase
The FastGene® T4 DNA Ligase catalyzes the formation of a covalent bond between the 5′-phosphate and 3′-OH in nicked duplex DNA or at two DNA ends. This activity is very useful to ligate DNA fragments with either cohesive or blunt ends, that are generated by restriction enzyme digestion. T4 DNA Ligase can also ligate RNA with DNA or RNA in a double helix with low efficiency. The T4 DNA Ligase is cloned and expressed in E. coli, and purified to homogeneity. The Ligase is free of endonuclease, exonuclease and phosphatase.
Lisätietoja
Lisätietoja
Lataukset / käyttöohjeet
Lataukset / käyttöohjeet
