{"product_id":"midori-green-direct-50µl-sample","title":"Midori Green Direct 50µl sample","description":"\u003cp\u003eMIDORI Green Direct is a safe alternative to ethidium bromide for visualization of double-stranded DNA, single-stranded DNA, and RNA in agarose gels. In contrast to most other non-ethidium bromide based dyes, MIDORIGreen Direct is just added to your samples. The loading dye is already included.  You do not need to add any other loading dye to both gel matrix and running buffers\u003c\/p\u003e\n\u003cp\u003eDirect Staining of DNA\/RNA\u003cbr\u003eNo toxicity, non-carcinogenic\u003cbr\u003eSafe alternative of Ethidium Bromide\u003cbr\u003eLoading Dye is included\u003cbr\u003eVery low Background\u003c\/p\u003e\n\u003cp\u003eGet the best signal to noise ratio\u003cbr\u003eThe direct staining of the DNA rather than the gel eliminates the background staining providing a perfect signal (Fig. 1). MIDORIGreen Direct was developed to work with Blue Light LED illuminators, but you can also use it with a regular UV transilluminator.\u003c\/p\u003e\n\u003cp\u003eBetter Results for Downstream Application\u003cbr\u003eThe isolation of DNA from agarose gels enables downstream applications. It is well known that many dyes, such as ethidium bromide or even SYBR™ Green are strong enzyme inhibitors due to their intercalating properties. MIDORI Green dyes bind to the DNA backbone. This results in a much higher efficiency for downstream applications, i.e. cloning (Fig. 2), seqeuncing, PCR, etc.\u003c\/p\u003e\n\u003cp\u003eProven Safety\u003cbr\u003eMIDORI Green Direct stain is non-carcinogenic and less mutagenic compared to ethidium bromide. Furthermore, we can state that MIDORI Green Direct is impenetrable to latex gloves and cell membranes.\u003c\/p\u003e\n\u003cp\u003eMIDORI Green Direct is classified as non-hazardous to aquatic life, under CCR Title 22 regulation. Thus, small amounts of MIDORI Green Direct stain can be safely released into the environment.\u003c\/p\u003e\n\u003cp\u003eAmes-Test\u003cbr\u003eCytotoxicity\u003cbr\u003eCell membrane Permeability\u003cbr\u003eHazardous Waste Screening\u003cbr\u003eLatex and Nitrile Gloves Penetration\u003c\/p\u003e\n\u003cp\u003eLoading Dye\u003cbr\u003eMIDORI Green Direct stains are provided in a form of 10X sample loading dyes and they are to be added to your samples only. You do not need to add any other dyes to the gel matrix nor to the running buffers.\u003c\/p\u003e","brand":"Nippon Genetics Europe GmbH","offers":[{"title":"Default Title","offer_id":54120107213097,"sku":"MG05","price":12.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0845\/9976\/7337\/files\/Tuotekuva_tulossa_23addb3e-11ff-4a01-9424-c05ea41e6855.png?v=1791742990","url":"https:\/\/shop.vingmed.fi\/products\/midori-green-direct-50%c2%b5l-sample","provider":"Vingmed","version":"1.0","type":"link"}