{"product_id":"fastgene-kickspeed-dna-ligation-kit-30-rxns","title":"FastGene Kickspeed DNA Ligation kit  30 rxns","description":"\u003cp\u003eKickspeed DNA Ligation Kit\u003cbr\u003eFastGene® Kickspeed DNA Ligation kit is formulated for quick ligation of DNA fragments with cohesive ends within 5 min at room temperature. This product uses T4 DNA Ligase and 2X Kickspeed DNA Ligation buffer.\u003c\/p\u003e\n\u003cp\u003eCharacteristics\u003c\/p\u003e\n\u003cp\u003e5 min ligation reaction with all types of DNA fragments\u003cbr\u003eReaction at room temperature\u003cbr\u003eRapid and efficient Kickspeed Ligation buffer\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eApplications\u003c\/p\u003e\n\u003cp\u003eGeneral cloning\u003cbr\u003eBlunt-end cloning\u003cbr\u003eTA cloning\u003cbr\u003eLibrary construction\u003cbr\u003eLinker ligation\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eT4 DNA Ligase\u003c\/p\u003e\n\u003cp\u003eThe FastGene® T4 DNA Ligase catalyzes the formation of a covalent bond between the 5′-phosphate and 3′-OH in nicked duplex DNA or at two DNA ends. This activity is very useful to ligate DNA fragments with either cohesive or blunt ends, that are generated by restriction enzyme digestion. T4 DNA Ligase can also ligate RNA with DNA or RNA in a double helix with low efficiency. The T4 DNA Ligase is cloned and expressed in E. coli, and purified to homogeneity. The Ligase is free of endonuclease, exonuclease and phosphatase.\u003c\/p\u003e","brand":"Nippon Genetics Europe GmbH","offers":[{"title":"Default Title","offer_id":53796927602985,"sku":"FG-LK30","price":57.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0845\/9976\/7337\/files\/Tuotekuva_tulossa_23addb3e-11ff-4a01-9424-c05ea41e6855.png?v=1791742990","url":"https:\/\/shop.vingmed.fi\/products\/fastgene-kickspeed-dna-ligation-kit-30-rxns","provider":"Vingmed","version":"1.0","type":"link"}