{"product_id":"fastgene-block-go","title":"FastGene Block \u0026 Go","description":"\u003cp\u003eFastGene\u003csup\u003e®\u003c\/sup\u003e Block \u0026amp; Go serves a \u003cstrong\u003eblocking solution\u003c\/strong\u003e as well as \u003cstrong\u003esignal enhancer\u003c\/strong\u003e in Western blot analysis.\u003cbr\u003eIt provides blocking and primary and secondary antibody hybridization in one step. Furthermore, it enhances the signal developed with HRP (horseradish peroxidase) or AP (alkaline phosphatase) substrates. FastGene\u003csup\u003e®\u003c\/sup\u003e Block \u0026amp; Go reduces time and labor-intensive Western bot procedure.\u003c\/p\u003e\n\u003ch4\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003e3 steps in one:\u003c\/strong\u003e blocking of the membrane, primary and secondary antibody in one step\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEnhanced antibody signal:\u003c\/strong\u003e 2-5-fold increase in signal intensity for most protein targets.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEnhance time-saving:\u003c\/strong\u003e It saves at least 2 hours in the antibody detection process during the Western Blot, with only one hour needed.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEffective with any ECL: \u003c\/strong\u003eAfter the antibody detection process, the signal can be developed with both HRP (horseradish peroxidase) and AP (alkaline phosphatase) substrates.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLess hands-on steps: \u003c\/strong\u003eNo 3 wash steps are required, meaning no need to transfer the membrane in\u0026amp;out of the container.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCompatible with PVDF \u0026amp; NC membrane:\u003c\/strong\u003e Regardless of the pore size, ‘Block \u0026amp; Go’ minimizes the background from non-specific protein binding by antibodies.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProtein free: \u003c\/strong\u003eReduces overall background and minimizes non-specific signals often seen with ECL detection.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eTime saving\u003c\/h4\u003e\n\u003cp\u003eBy combining 3 steps in one step (blocking + primary antibody + secondary antibody), the procedure can be reduced about 1 h (2-step procedure) or even 2.5 h (1-step procedure) compared to conventional protocol using milk or BSA.\u003c\/p\u003e\n\u003ch4\u003eEnhanced signal intensity\u003c\/h4\u003e\n\u003cp\u003eDetection sensitivity can be enhanced so that proteins from 0.5 µg lysate can be detected. \u003c\/p\u003e","brand":"Nippon Genetics","offers":[{"title":"Default Title","offer_id":49216452133161,"sku":"FG-CH05","price":0.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0845\/9976\/7337\/files\/FG-CH05_FastGeneBlock_Nippon_Genetics.png?v=1788797331","url":"https:\/\/shop.vingmed.fi\/en\/products\/fastgene-block-go","provider":"Vingmed","version":"1.0","type":"link"}