{"product_id":"bluestar-prestained-protein-marker","title":"BlueStar Prestained Protein Marker 500µl","description":"\u003ch4\u003eBlueStar Prestained Protein Marker\u003c\/h4\u003e\n\u003cp\u003eThe BlueStar Prestained Protein Ladder is a three color protein standard with 10 prestained proteins covering a wide range molecular weights from 10 to 180 kDa. The BlueStar Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon or nitrocellulose) and for approximating the size of proteins.\u003c\/p\u003e\n\u003cdiv class=\"wc-section entry-content\" id=\"section-details\"\u003e\n\u003cul\u003e\n\u003cli\u003eBroad range: 10 – 180 kDa\u003c\/li\u003e\n\u003cli\u003eHigh performance: 10 bands with 3 colours (blue, orange and green)\u003c\/li\u003e\n\u003cli\u003eReference bands: 28 kDa (green) and 75 kDa (orange)\u003c\/li\u003e\n\u003cli\u003eSharp bands\u003c\/li\u003e\n\u003cli\u003eReady to use: Supplied in a loading buffer for direct loading\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eStorage Buffer\u003c\/h4\u003e\n\u003cp\u003e20 mM Disodium hydrogen phosphate pH 8.0 (at 25°C ), 2 % SDS, 0.2% Triton X-100,10 mM 2-Mercaptoethanol, 6 M Urea and 27% (v\/v) Glycerol.\u003c\/p\u003e\n\u003ch4\u003eStorage\u003c\/h4\u003e\n\u003cp\u003eStable at -20°C for 12 months.\u003cbr\u003eStable at +4°C for 3 months.\u003c\/p\u003e\n\u003ch4\u003eRecommendations for Loading\u003c\/h4\u003e\n\u003cp\u003e1. Thaw the ladder either at room temperature or at 37-40°C for a few minutes to dissolve precipitated solids. Do not boil.\u003cbr\u003e2. Mix thoroughly, to ensure that the solution is homogeneous.\u003cbr\u003e3. Load the following volumes of the ladder on SDS-polyacrylamide gel:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e5 μl per well for mini-gels, 3 μl per well for blots\u003c\/li\u003e\n\u003cli\u003e10 μl per well for large gels, 6 μl per well for blots\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"wc-section entry-content\" id=\"section-applications\"\u003e\u003c\/div\u003e","brand":"Nippon Genetics Europe GmbH","offers":[{"title":"Default Title","offer_id":49216663716137,"sku":"MWP03","price":152.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0845\/9976\/7337\/files\/Tuotekuva_tulossa_23addb3e-11ff-4a01-9424-c05ea41e6855.png?v=1791742990","url":"https:\/\/shop.vingmed.fi\/en\/products\/bluestar-prestained-protein-marker","provider":"Vingmed","version":"1.0","type":"link"}