{"product_id":"bluestar-plus-prestained-protein-marker","title":"BlueStar Plus Prestained Protein Marker 500µl","description":"\u003ch4\u003eBlueStar PLUS Prestained Protein Marker\u003c\/h4\u003e\n\u003cp\u003eThe BlueStar PLUS Prestained Protein Marker is a three color protein standard with 12 prestained proteins covering a wide range molecular weights from 10 – 240 kDa.\u003cbr\u003eThe Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins.\u003c\/p\u003e\n\u003ch5\u003eContents\u003c\/h5\u003e\n\u003cp\u003eApproximately 0.1~0.4 mg\/ml of each protein in the buffer (20 mM Tris-phosphate, 2 % SDS, 0,2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v\/v) Glycerol).\u003c\/p\u003e\n\u003cp\u003e500 µL Protein Marker sufficient for 100 mini gels or 50 large gels.\u003c\/p\u003e\n\u003ch4\u003eStorage\u003c\/h4\u003e\n\u003cp\u003eStore at -20°C for 24 months.\u003cbr\u003eStable at +4°C for 3 months.\u003cbr\u003eStable at +25°C for 2 weeks.\u003c\/p\u003e\n\u003ch4\u003eRecommendations for Loading\u003c\/h4\u003e\n\u003col\u003e\n\u003cli\u003eThaw the ladder either at room temperature or at 37-40°C for a few minutes to dissolve precipitated solids. Do not boil.\u003c\/li\u003e\n\u003cli\u003eMix thoroughly, to ensure that the solution is homogeneous.\u003c\/li\u003e\n\u003cli\u003eLoad the following volumes of the ladder on SDS-polyacrylamide gel:\u003cbr\u003e– 5 μl per well for mini-gels, 3 μl per well for blots;\u003cbr\u003e– 10 μl per well for large gels, 6 μl per well for blots\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Nippon Genetics Europe GmbH","offers":[{"title":"Default Title","offer_id":49216706216233,"sku":"MWP04","price":161.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0845\/9976\/7337\/files\/MWP04_BlueStarPlusPrestainedProteinMarker500_l.png?v=1788807676","url":"https:\/\/shop.vingmed.fi\/en\/products\/bluestar-plus-prestained-protein-marker","provider":"Vingmed","version":"1.0","type":"link"}