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Optional supplementation of Non-Essential Amino Acids (NEAA's) to the formulations that incorporate either Hank's or Eagle's salts has broadened the utility of this medium.\u003c\/p\u003e\n\u003cp\u003eMEM is simply a modification BME containing higher concentrations of essential nutrients. MEM has been utilized for the cultivation of a wide array of cells grown in monolayers. The optional supplementation of Non-Essential Amino Acids (NEAA's) to the formulations that incorporate either Hank's or Eagle's Salts has broadened the usefulness of this medium. Subsequent studies by Eagle and other researchers determined that if additions were made to the original BME, it could aid and support a much wider spectrum of more fastidious cells in culture. MEM incorporates these modifications and thus includes, among others constituents, higher concentrations of Amino Acids so as to more closely approximate the protein composition of Mammalian cells in culture. 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