{"title":"PCR -reagenssit","description":"\u003cp\u003e1- ja 2-step kitit SYBR- ja Probe-pohjaiselle qPCR:lle ja erilaisilla end-point PCR-polymeraaseilla. Lisäksi NGS-kittejä kirjastojen valmisteluun ja kvantifiointiin ja magneettihelmiä puhdistusta varten.\u003c\/p\u003e","products":[{"product_id":"mytaq™-hs-red-mix","title":"MyTaq HS Red Mix, 50µl 200rxns\/pk","description":"\u003cp\u003e\u003cstrong\u003eMyTaq™ HS Red Mix \u003c\/strong\u003eis recommended for PCR assays containing complex and low copy number targets as well as multiplex PCR. MyTaq HS Red Mix is comprised of MyTaq HS DNA Polymerase and a novel buffer system that deliver very high yield PCR amplification over a wide range of PCR templates. MyTaq HS has an increased affinity for DNA, enabling reliable amplification from even very low amounts of template. MyTaq HS has been developed to give more robust amplification than other commonly-used polymerases allowing it to perform well with challenging templates and in the presence of PCR inhibitors. Furthermore, the highly efficient nature of MyTaq HS means it gives excellent results under fast PCR conditions. MyTaq HS does not possess polymerase activity during the reaction set-up, thereby reducing the non-specific amplification that can hinder PCR assays from the start.\u003c\/p\u003e\n\u003cp\u003eThe product is supplied as a mastermix that requires the addition of only template, primers and water, thereby reducing the number of pipetting steps during PCR set-up for improved speed, throughput and assay reproducibility. The inclusion of dNTPs, MgCl\u003csub\u003e2\u003c\/sub\u003e\u003cspan\u003e \u003c\/span\u003eand enhancers at optimal concentrations, helps eliminate the need for optimization, thereby saving time and ensuring comparable efficiencies for annealing and extension of all primers in the reaction, this makes MyTaq HS Red Mix ideal for multiplex PCR.\u003c\/p\u003e\n\u003cp\u003eMyTaq HS Red Mix, includes a red dye that increases the visual contrast between the reagent and the reaction vessel for improved convenience and to improve pipetting accuracy. The red dye also enables samples to be loaded directly on to a gel after the PCR eliminating the need to add loading buffer.\u003c\/p\u003e","brand":"Bioline Reagents Limited (Meridian Life Science)","offers":[{"title":"2x 200 - 800 reactions","offer_id":49730114552105,"sku":"BIO-25047","price":165.0,"currency_code":"EUR","in_stock":true},{"title":"2x 1000 - 4000 reactions","offer_id":49730114584873,"sku":"BIO-25048","price":732.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0845\/9976\/7337\/files\/BIO-25048_MyTaqHSRedMix.png?v=1777194995"},{"product_id":"sensifast™-cdna-synthesis-kit","title":"SensiFAST cDNA Synthesis Kit 50rxns\/pk","description":"\u003cp\u003eThe SensiFAST™ cDNA Synthesis Kit provides a rapid and sensitive method for first-strand cDNA synthesis, which displays excellent linearity across a wide range of starting material. This gives the same relative representation in cDNA templates, regardless of gene abundance, making it excellent for use in qPCR studies.\u003c\/p\u003e\n\u003cp\u003eSensiFAST cDNA Synthesis Kit contains a highly-pure reverse transcriptase and optimized TransAmp™ buffer system, which includes a unique blend of random hexamers and anchored oligo (dT) primers to deliver the highest quality qPCR ready cDNA. This makes the SensiFAST cDNA Synthesis Kit ideal for working with limited sample volumes, such as laser-micro dissected samples and tissue biopsies (down to 1 pg of input RNA), to reverse transcribe precious RNA into stable cDNA ready for accurate real-time quantification. The unique blend of random hexamer primers and anchored oligo dT in the TransAmp Buffer also ensure unbiased 3’ and 5’ coverage and reverse transcription of all regions.\u003c\/p\u003e\n\u003cp\u003eSensiFAST cDNA Synthesis Kit can be used with SensiFAST Probe and SensiFAST SYBR\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eKits for fast real-time RT-qPCR without compromising on quality, giving real-time results in less than an hour.\u003c\/p\u003e","brand":"Bioline Reagents Limited (Meridian Life Science)","offers":[{"title":"50 Reactions","offer_id":49730131558697,"sku":"BIO-65053","price":234.0,"currency_code":"EUR","in_stock":true},{"title":"250 Reactions","offer_id":49730131591465,"sku":"BIO-65054","price":711.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0845\/9976\/7337\/files\/BIO-65053SensiFASTcDNASynthesisKit50Reactions.png?v=1764193921"}],"url":"https:\/\/shop.vingmed.fi\/collections\/pcr-reagenssit.oembed","provider":"Vingmed","version":"1.0","type":"link"}